EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK) | US Biological E2215-30B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

catalog :

E2215-30B

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

9j16(15F7)

reactivity :

human

product information

Catalog Number :

E2215-30B

Product wo Prefix :

EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

9j16(15F7)

Isotype :

IgG

Desc1 :

EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3). Applications: Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunofluorescence (IF-IC): 1:800 Immunohistochemistry (Paraffin): 1:100 Immunoprecipitation: 1:50 Chromatin Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to the N-terminal sequence of human EGR1. Species Sequence Homology: Bovine.

Specificity :

Recognizes endogenous levels of total human EGR1 at ~75kD. Species Crossreactivity: Mouse, rat.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 9j16(15F7) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the N-terminal sequence of human EGR1. Species Sequence Homology: Bovine. Specificity: Recognizes endogenous levels of total human EGR1 at ~75kD. Species Crossreactivity: Mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

ChIP IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. O’Donovan, K.J., et al., (1999) Trends Neurosci. 22:167–173. 2. Harada, T., et al., (2001) Nat. Cell Biol. 3:453–459. 3. Papanikolaou, N.A. & Sabban, E.L., (2000) J. Biol. Chem. 275: 26,683–26,689.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/E2215-30B_1.jpg

Picture4 Text :

Immunohistochemical staining of human non-Hodgkin’s lymphoma using E2215-30B.

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/E2215-30B_5.jpg

Picture5 Text :

Immunohistochemical analysis of paraffin-embedded mouse brain, using E2215-30B.