EDG1 (Endothelial Cell Differentiation Gene 1) (APC) | US Biological E2208-01D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

EDG1 (Endothelial Cell Differentiation Gene 1) (APC)

catalog :

E2208-01D

quantity :

100Tests

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

8H120

reactivity :

human

product information

Catalog Number :

E2208-01D

Product wo Prefix :

EDG1 (Endothelial Cell Differentiation Gene 1) (APC)

Host :

mouse

Product Type :

Mab

Conjugate :

APC

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

8H120

Isotype :

IgG2b

Desc1 :

EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG-1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 human EDG1/S1P1-transfected CHO-S cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, 10ul of E2208-01D was added to up to 1x10e6 cells and incubated for 30 minutes at RT. 3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. The cells were resuspended in F4568-10 for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2b antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to aa1-382 from human EDG1 expressed in NS0 cells.

Specificity :

Recognizes human EDG1.

Purity :

Purified by Protein A affinity chromatography from hybridoma tissue culture supernatant.

Form :

Supplied as a liquid in saline, ≤0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).

Concentration :

~0.01mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 8H120 Host: mouse Source: human Concentration: ~0.01mg/ml Form: Supplied as a liquid in saline, ≤0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC). Purity: Purified by Protein A affinity chromatography from hybridoma tissue culture supernatant. Immunogen: Recombinant protein corresponding to aa1-382 from human EDG1 expressed in NS0 cells. Specificity: Recognizes human EDG1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do not freeze

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/1/E2208-01D_1.jpg