Dynamin 1,2 | US Biological D9900-50 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Dynamin 1,2

catalog :

D9900-50

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

D9900-50

Product wo Prefix :

Dynamin 1,2

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Dynamin is a family of large GTPases that has been implicated in the formation of nascent vesicles of both endocytotic and secretory processes. Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the responsiveness of signal transduction. It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCgamma, PKC and G-proteins. Dynamin is phosphorylated by PKC and Src. Its phosphorylation may regulate the endocytosis of cell surface receptors. Applications: Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:30 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Tyr231 of human dynamin, conjugated to KLH

Specificity :

Recognizes endogenous levels of human total dynamin protein. Does not recognize the related dynamin-like protein DLP. Species Crossreactivity: mouse and rat

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

~0.8mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.8mg/ml Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Tyr231 of human dynamin, conjugated to KLH Specificity: Recognizes endogenous levels of human total dynamin protein. Does not recognize the related dynamin-like protein DLP. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. McNiven, M.A., et al., Trends Biochem. Sci. 25: 115-120 (2000). 2. Powell, K.A., et al., J. Biol. Chem. 275: 11610-11617 (2000). 3. Ahn, S., et al., J. Biol. Chem. 274: 1185-1188 (1999).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/10/D9900-50_1.jpg