Digoxigenin discontinued | US Biological D8029-50A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Digoxigenin discontinued

catalog :

D8029-50A

quantity :

100 ug

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

product information

Catalog Number :

D8029-50A

Product wo Prefix :

Digoxigenin discontinued

Host :

chicken

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgY

Desc1 :

Digoxigenin is considered a hapten, a small molecule with high immunigenicity used in many molecular biology applications. Other popular haptens include DNP (dinitrophenol), biotin and fluorescein. In general, antibodies generated against haptens have higher affinities for their targets than other antibodies, so haptens are conjugated to other biological molecules as all-purpose immuno-tags. This is because anti-hapten antibodies have high affinities and are readily available, while antibodies may not be commercially available for exotic biological targets. Digoxigenin is a standard immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically Uridine), which is then incorporated into RNA (a "riboprobe") as it is synthesized by the cellular machinery. Later, the Digoxigenin ("Dig") markers are detected with anti-digoxigenin antibodies, which may be labeled with dyes or enzymes (see ELISA) for visualization and detection. In a similar manner, Digoxigenin may be conjugated to sugars to study glycosylation events in biological systems. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Digoxigenin (KLH)

Specificity :

Recognizes Digoxigenin.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgY Host: chicken Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Digoxigenin (KLH) Specificity: Recognizes Digoxigenin. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C