DNA | US Biological D3875-01 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

DNA

catalog :

D3875-01

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AC-30-10

product information

Catalog Number :

D3875-01

Product wo Prefix :

DNA

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

AC-30-10

Isotype :

IgM

Desc1 :

This clone is a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. It can also be used for sensitive detection of mycoplasma contamination in cell cultures. Applications: Suitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue) and Dot blot on nitrocellulose membrane (after baking at 70°C). Recommended Dilution: Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1hr at RT. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Double- and single-stranded DNA.

Specificity :

Reactivities on cultured XLKE-A6 (Xenopus), RVF-SMC, cell lines (tested so far) PTK-2, HeLa species (tested so far). Binding characteristics: Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA. Antigen recognized in All DNA-containing species.

Purity :

Gel filtration.

Form :

Supplied as a lyophilized powder

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgM Clone No: AC-30-10 Host: mouse Concentration: ~0.1mg/ml Form: Supplied as a lyophilized powder. Purity: Gel filtration. Immunogen: Double- and single-stranded DNA. Specificity: Reactivities on cultured XLKE-A6 (Xenopus), RVF-SMC, cell lines (tested so far) PTK-2, HeLa species (tested so far). Binding characteristics: Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA. Antigen recognized in All DNA-containing species. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

DB IHC

Storage Temperature :

-20°C

Reference :

Benavente, R. and Krohne, G.: Involvement of nuclear lamins in post mitotic reorganisation of chromatin as demonstrated by microinjection of lamin antibodies. J. Cell Biol. 103, 1847-1854 (1986) Hausman, P., Falk, H., Scheer, U. and Sitte, P.: Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA antibody. Eur. J. Cell Biol. 42, 152-160 (1986) Scheer, U.: Structure of lampbrush chromosome loops during different states of transcriptional activity as visualized in the presence of physiological salt concentrations. Biol. Cell 59, 33-42 (1987) Scheer, U., Messner, K., Hazan, R., Raska, I., Hausmann, P., Falk, H., Spiess, E. and Franke, W.W.: High sensitivity immunolocalization of double- and single-stranded DNA by a monoclonal antibody. Eur. J. Cell Biol. 43, 358-371 (1987)