antibody

Desmin (DES, CMD1I, CSM1, CSM2, Intermediate Filament Protein)

D3221-02E

100 ul

monoclonal

domestic rabbit

nonconjugated

10E18(D93F5)

Desmin (DES, CMD1I, CSM1, CSM2, Intermediate Filament Protein)

rabbit

Mab

Antibodies

100 ul

10E18(D93F5)

IgG

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments and microtubules. Major types of intermediate filaments are distinguished and expressed in particular cell types: cytokeratins (epithelial cells), glial fibrillary acidic protein or GFAP (glial cells), desmin (skeletal, visceral and certain vascular smooth muscle cells), vimentin (mesenchymeorigin) and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes. Vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers to distinguish between the two forms of neoplasm. Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs. The desmin cytoskeleton provides a connection among myofibrils, organelles and the cytoskeleton. Desmin knockout mice develop cardiomyopathy, skeletal and smooth muscle defects. In humans, desmin related myopathies might be caused by mutations in the corresponding desmin gene or in proteins with which desmin interacts, including αB-crystallin and synemin. Disorganized desmin filaments and the accumulation of protein aggregates comprised predominantly of desmin characterize desmin-related myopathies. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:100 Immunofluorescence (IC): 1:100 Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide corresponding to the C-terminal residues of human desmin protein. Species Sequence Homology: monkey, 100%

Recognizes endogenous levels of total human desmin protein at ~53kD. Species Crossreactivity: mouse and rat

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 10E18(D93F5) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to the C-terminal residues of human desmin protein. Species Sequence Homology: monkey, 100% Specificity: Recognizes endogenous levels of total human desmin protein at ~53kD. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IC IF WB

-20°C

1. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51, 2. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93, 3. Leader, M. et al. (1987) Histopathology 11, 63-72, 4. Capetanaki, Y. et al. (2007) Exp Cell Res 313, 2063-76, 5. Li, Z. et al. (1996) Dev Biol 175, 362-6, 6. Paulin, D. and Li, Z. (2004) Exp Cell Res 301, 1-7, 7. Paulin, D. et al. (2004) J Pathol 204, 418-27.

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