antibody

Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein)

D3221-02B

100 ug

polyclonal

domestic goat

nonconjugated

Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, intermediate filament protein)

goat

Pab

Antibodies

100 ug

IgG

Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomysarcomas (smooth muscle) as well as rhabdomysarcomas (striated muscle). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:4000 Western Blot: 0.1-0.3ug/ml ~55-60kD band observed in human, mouse, rat and porcine Skeletal Muscle lysates (calculated MW of 53.5kD according to NP_001918.3). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See D3221-02B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Hu Mo Po Rt

DES; desmin; CMD1I; CSM1; CSM2; FLJ12025; FLJ39719; FLJ41013; FLJ41793; intermediate filament protein

Synthetic peptide corresponding to the C-terminus of RDGEVVSEATQQQHE. Species sequence homology: canine, porcine and bovine

Recognizes human Desmin. Species Crossreactivity: mouse, rat and porcine

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

~0.5mg/ml

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminus of RDGEVVSEATQQQHE. Species sequence homology: canine, porcine and bovine Specificity: Recognizes human Desmin. Species Crossreactivity: mouse, rat and porcine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/15/D3221-02B_1.jpg