Delta 4 (Delta-like Protein 4, DLL4, Drosophila Delta Homolog 4, Notch Ligand DLL4 Precursor) | US Biological D2728-01 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Delta 4 (Delta-like Protein 4, DLL4, Drosophila Delta Homolog 4, Notch Ligand DLL4 Precursor)

catalog :

D2728-01

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

D2728-01

Product wo Prefix :

Delta 4 (Delta-like Protein 4, DLL4, Drosophila Delta Homolog 4, Notch Ligand DLL4 Precursor)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). DLL4 expression is highly restricted to the vascular endothelium (7), and haploinsufficiency of DLL4 results in major defects in vascular systems in mouse (8-11). Blockade of DLL4 inhibits tumor growth in model systems (12-14). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to a region surrounding residue Leu617 of human Delta-like 4 (DLL4) (KLH).

Specificity :

Recognizes endogenous levels of total human DLL4 protein at ~80kD.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to a region surrounding residue Leu617 of human Delta-like 4 (DLL4) (KLH). Specificity: Recognizes endogenous levels of total human DLL4 protein at ~80kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860–2868. 2. Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320–328. 3. Chiba, S. (2006) Stem Cells 24, 2437–2447. 4. Bland, C.E. et al. (2003) J. Biol. Chem. 278, 1360–13610. 5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638–7643

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/11/D2728-01_1.jpg