Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172) | US Biological D2727-75 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

catalog :

D2727-75

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H56

reactivity :

human

product information

Catalog Number :

D2727-75

Product wo Prefix :

Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

7H56

Isotype :

IgG2b

Desc1 :

Delta-like protein 1 (DLL1) is a 90-100kD type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23aa transmembrane segment and a 155aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37% and 54% aa sequence identity with DLL2, 3 and 4, respectively. A 60kD ECD fragment released by ADAM9, 12 or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13). Applications: Suitable for use in ELISA, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml detects DLL1 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Immunohistochemistry: 8-25ug/ml detects DLL1 in immersion fixed paraffin embedded human squamous cell carcinoma tissue sections. Optimal dilutions should be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Ser22-Gly540 from human Delta-like 1 expressed in NSO cells (Accession #AAG09716).

Specificity :

Recognizes recombinant human DLL1 in direct ELISAs. Does not crossreact with recombinant human DLL3 or recombinant human DLL4.

Purity :

Purified by Protein G affinity chromatography from cell culture supernatant.

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 7H56 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein G affinity chromatography from cell culture supernatant. Immunogen: Recombinant protein corresponding to Ser22-Gly540 from human Delta-like 1 expressed in NSO cells (Accession #AAG09716). Specificity: Recognizes recombinant human DLL1 in direct ELISAs. Does not crossreact with recombinant human DLL3 or recombinant human DLL4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC IHC

Storage Temperature :

-20°C

Reference :

1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 3. Karanu, F.N. et al. (2001) Blood 97:1960. 4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 6. Takahashi, Y. et al. (2003) Development 130:4259. 7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68. 8. Kiernan, A.E. et al. (2005) Development 132:4353. 9. Schmitt, T.M. and J.C. ZunigaPflucker (2002) Immunity 17:749. 10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638. 11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454. 12. Limbourg, A. et al. (2007) Circ. Res. 100:363. 13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/37/D2727-75_1.jpg