Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172) (APC) | US Biological D2727-70C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172) (APC)

catalog :

D2727-70C

quantity :

100Tests

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

9H109

reactivity :

human

product information

Catalog Number :

D2727-70C

Product wo Prefix :

Delta-like 1 (DLL1, Delta1, DL1, H-Delta-1, UNQ146/PRO172) (APC)

Host :

mouse

Product Type :

Mab

Conjugate :

APC

Category :

Antibodies

Size1 :

100Tests

Clone # USB :

9H109

Isotype :

IgG2b

Desc1 :

DLL1 is the human homolog of the Drosophila Notch ligand, Delta. It is a type I transmembrane protein with a DSL (Delta, Serrate, Jagged) domain followed by 8 EGF-like domains in its extracellular region and a short cytoplasmic region. Notch-DSL signaling plays an important role in the differentiation processes of many tissues. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6. T95G human Glioblastoma cell line Optimal dilutions to be determined by the researcher. Flow Cytometry: 1. T98G cells. may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, add 10ul of D2727-70C to ≤1x10e6 cells and incubated for 30 minutes at RT. 3. Wash the cells twice in F4568-10 Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspended the cells in F4568-10 Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. This procedure may need to be modified, depending upon the cell type and final utilization. Individual users may need to titrate to determine the optimal reagent amount for their specific use. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Ser22-Gly540 of human Delta Like Protein 1 extracelular domain expressed in NSO cells.

Specificity :

Recognizes human Delta-like 1. Does not crossreact with recombinant mouse DLL4.

Purity :

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Form :

Supplied as a liquid in saline buffer, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).

Concentration :

~0.01mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 9H109 Host: mouse Source: human Concentration: ~0.01mg/ml Form: Supplied as a liquid in saline buffer, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC). Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant. Immunogen: Recombinant protein corresponding to Ser22-Gly540 of human Delta Like Protein 1 extracelular domain expressed in NSO cells. Specificity: Recognizes human Delta-like 1. Does not crossreact with recombinant mouse DLL4. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

4°C Do Not Freeze

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/0/D2727-70C_1.jpg