DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, G17P1, HELR, HLR1) | US Biological D1660-40J product information

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product summary

company name :

US Biological

product type :

antibody

product name :

DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, G17P1, HELR, HLR1)

catalog :

D1660-40J

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

product information

Catalog Number :

D1660-40J

Product wo Prefix :

DDX5 (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, G17P1, HELR, HLR1)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

DDX5, also known as p68 RNA helicase, is a 614aa, 68kD member of the DEAD box family of RNA helicases that contain the conserved Asp-Glu-Ala-Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX-17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX-17 homology region. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:64,000 Western Blot: 0.11-0.3ug/ml, observed in human epidermoid carcinoma A431 lysates on ~70kD bands Immunohistochemistry (Paraffin): 2-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ca Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to C-PMIGYPMPTGYSQ, from human DDX5, at C-terminal (NP_004387.1).

Specificity :

Recognizes human DDX5. Species sequence homology: Canine, mouse and rat.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-PMIGYPMPTGYSQ, from human DDX5, at C-terminal (NP_004387.1). Specificity: Recognizes human DDX5. Species sequence homology: Canine, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Fukuda T, Yamagata K, Fujiyama S, Matsumoto T, Koshida I, Yoshimura K, Mihara M, Naitou M, Endoh H, Nakamura T, Akimoto C, Yamamoto Y, Katagiri T, Foulds C, Takezawa S, Kitagawa H, Takeyama K, O'Malley BW, Kato S. DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs. Nat Cell Biol. 2007 May;9(5):604-11.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/18/D1660-40J_1.jpg