antibody

Daxx (BING2, DAP 6, DAP6, Death associated protein 6, EAP 1, EAP1, ETS1 associated protein 1, Fas death domain associated protein, hDaxx, MGC126245, MGC126246)

D1150-03

100 ul

monoclonal

domestic rabbit

nonconjugated

25C12

The same clone is also sold as:

• Cell Signaling Technology: 4533

Daxx (BING2, DAP 6, DAP6, Death associated protein 6, EAP 1, EAP1, ETS1 associated protein 1, Fas death domain associated protein, hDaxx, MGC126245, MGC126246)

rabbit

Mab

Antibodies

100 ul

25C12

IgG

Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (7). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-kB and E2F-1 (8). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (Immunocytochemistry): 1:25 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide corresponding to a region surrounding Gln255 of Daxx. Species Sequence Homology: Bovine, canine and monkey (100%)

Detects endogenous levels of total Daxx protein. Species Crossreactivity: Human, mouse and rat.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 25C12 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to a region surrounding Gln255 of Daxx. Species Sequence Homology: Bovine, canine and monkey (100%) Specificity: Detects endogenous levels of total Daxx protein. Species Crossreactivity: Human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF WB

-20°C

1 Yang, X. et al. (1997) Cell 89, 1067-1076. 2 Torii, S. et al. (1999) EMBO J. 18, 6037-6049. 3 Li, H. et al. (2000) Mol. Cell Biol. 20, 1784-1796. 4 Pluta, A.F. et al. (1998) J. Cell Sci. 111, 2029-2041. 5 Hollenbach, A.D. et al. (1999) EMBO J. 18, 3702-3711. 6 Michaelson, J.A. et al. (1999) Gene Dev. 13, 1918-1923. 7 Hollenbach, A.D. et al. (2002) J. Cell Sci. 115, 3319-3330. 8 Michaelson, J.A. and Leder, P. (2003) J. Cell Sci. 116, 345-352.

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