antibody

Death-associated protein kinase (DAPK1)

D1051-02

100 ul

polyclonal

domestic rabbit

nonconjugated

Death-associated protein kinase (DAPK1)

rabbit

Pab

Antibodies

100 ul

IgG

Death-associated protein kinase (DAPK1) is a Ca2+/calmodulin-regulated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-gamma, tumor necrosis factor alpha, Fas, activated c-Myc, and detachment from the extracellular matrix. In addition to the kinase domain and calmodulin regulatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain (1-3). Deletion of the calmodulin-regulatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-gamma-induced cell death (4). The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1 (5). Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia (6,7). Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo

Synthetic peptide corresponding to residues surrounding Gly903 of human DAPK1. Species sequence homology: rat and monkey (100%)

Recognizes endogenous levels of total human DAPK1 protein. MW:160kD Species Crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Gly903 of human DAPK1. Species sequence homology: rat and monkey (100%) Specificity: Recognizes endogenous levels of total human DAPK1 protein. MW:160kD Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

(1) Kimchi, A. (1999) Ann Rheum Dis. 58, I14–I19. (2) Cohen, O. et al. (1999) J Cell Biol 146, 141–148. (3) Deiss, L. P. et al. (1995) Genes Dev 9, 15–30. (4) Cohen, O. et al. (1997) EMBO J 16, 998–1008. (5) Velentza, A. V. et al. (2001) J Biol Chem 276, 38956–38965. (6) Raval, A. et al. (2007) Cell 129, 879–890. (7) Katzenellenbogen, R.A. et al. (1999) Blood 93, 4347–4353.

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