antibody

Cyclin B1, phosphorylated (Ser133)

C8453-04D

100 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

8A11(9E3)

Cyclin B1, phosphorylated (Ser133)

rabbit

Mab

Phosphorylated

Antibodies

100 ul

8A11(9E3)

IgG

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15. The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events. Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint. Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation. While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Ser133 of human cyclin B1.

Recognizes endogenous levels of human cyclin B1 protein only when phosphorylated at Ser133 at ~55kD.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 8A11(9E3) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser133 of human cyclin B1. Specificity: Recognizes endogenous levels of human cyclin B1 protein only when phosphorylated at Ser133 at ~55kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

(1. Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. 2. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675–1685. 3. Watanabe, N. et al. (1995) EMBO J. 14, 1878-1891. 4. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-058. 5. Hunter, T. et al. (1995) Cell 80, 225-236. 6. Diehl, J.A. et al. (1997) Genes Dev. 11, 957-972. 7. McGowan,C.H. et al.(1993) EMBO J. 12, 75-85. 8. Izumi, T. et al. (1991) Mol. Cell. Biol. 11, 3860-3867. 9. Li, J. et al. (1995) Mol. Biol. Cell 6, 1111-1124. 10. Li, J. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 502-507. 11. Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-220. 12. Peter, M. et al. (2002) EMBO Rep. 3, 551-556. 13. Jackman, M. et al. (2003)Nat. Cell Biol. 5, 143-148.

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