antibody

Cyclin B1

C8453-02

100 ul

polyclonal

domestic rabbit

nonconjugated

Cyclin B1

rabbit

Pab

Antibodies

100 ul

IgG

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13). Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hm Hu Mk Mo Rt

Syntheic peptide corresponding to residues near the N-terminus of human Cyclin B1.

Detects endogenous levels of total Cyclin B1. Species Crossreactivity: hamster, mouse, rat and monkey

Purified using peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

As reported

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified using peptide affinity chromatography. Immunogen: Syntheic peptide corresponding to residues near the N-terminus of human Cyclin B1. Specificity: Detects endogenous levels of total Cyclin B1. Species Crossreactivity: hamster, mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF WB

-20°C

(1) Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem.61, 441–470. (2) Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13,1675–1685. (3) Watanabe, N. et al. (1995) EMBO J. 14, 1878–1891. (4) Galaktionov, K. et al. (1995) Genes Dev. 9, 1046–1058. (5) Hunter, T. et al. (1995) Cell 80, 225–236. (6) Diehl, J.A. et al. (1997) Genes Dev. 11, 957–972. (7) McGowan, C.H. et al. (1993) EMBO J. 12, 75–85. (8) Izumi, T. et al. (1991) Mol. Cell. Biol. 11, 3860–3867. (9) Li, J. et al. (1995) Mol. Biol. Cell 6, 1111–1124. (10) Li, J. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 502–507. (11) Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215–220. (12) Peter, M. et al. (2002) EMBO Rep. 3, 551–556. (13) Jackman, M. et al. (2003) Nat. Cell Biol. 5, 143–148.

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