Csk (C-Terminal Src kinase, c-src Tyrosine kinase) | US Biological C7945-03 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Csk (C-Terminal Src kinase, c-src Tyrosine kinase)

catalog :

C7945-03

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

C74C1

The same clone is also sold as:

Cell Signaling Technology: 4980

reactivity :

human

product information

Catalog Number :

C7945-03

Product wo Prefix :

Csk (C-Terminal Src kinase, c-src Tyrosine kinase)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

C74C1

Isotype :

IgG

Desc1 :

Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine (1,2). Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK (3). This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk (4). Csk consists of an SH2, an SH3, and a kinase domain. There is evidence that the SH2 and SH3 domains are essential for the regulation of SFK, and Csk can be recruited to the membrane where SFKs are in an active state. This process is mediated by a Csk-binding protein (Cbp, also called PAG), which binds tightly to the SH2 domain of Csk (5). Activation of SFK by extracellular stimuli leads to the tyrosine phosphorylation of Cbp, generating docking sites for Csk. The recruitment of Csk forms a feedback mechanism for termination of SFK function (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Po Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding amino acid residue Val399 of human Csk.

Specificity :

Detects endogenous levels of total human Csk protein. Species Crossreactivity: mouse, rat, monkey and porcine

Purity :

As reported

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: C74C1 Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide. Purity: As reported Immunogen: Synthetic peptide corresponding to residues surrounding amino acid residue Val399 of human Csk. Specificity: Detects endogenous levels of total human Csk protein. Species Crossreactivity: mouse, rat, monkey and porcine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Nada, S. et al. (1991) Nature 351, 69–72. (2) Nada, S. et al. (1993) Cell 73, 1125–1135. (3) Lee, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14707–14712. (4) Imamoto, A. and Soriano, P. (1993) Cell 73, 1117–1124. (5) Kawabuchi, M. et al. (2000) Nature 404, 999–1003. (6) Matsuoka, H. et al. (2004) J. Biol. Chem. 279, 5975–5983.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/C7945-03_1.jpg