antibody

CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

C7925-10

100 ul

monoclonal

mouse

nonconjugated

3i10(32H4)

CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

mouse

Mab

Antibodies

100 ul

3i10(32H4)

IgG1

CrkL is a 39kD adaptor protein, originally cloned in proximity to the BCR gene on chromosome 22, which has a key regulatory role in hematopoietic cells. CrkL has one SH2 and two SH3 domains, with 60% homology to CrkII (1). Structurally, the amino-terminal SH3 domain of CrkL has been shown to bind proteins such as C3G, SOS, PI3K, c-Abl or BCR/Abl. The SH2 domain of CrkL can bind to tyrosinephosphorylated proteins such as Cbl, HEF1, CAS or paxillin (2,3). CrkL is involved in various signalings initiated by different cytokines and growth factors. The biological outcomes of the Crk-activated signal transduction cascades include the modulation of cell adhesion, cell migration, immune cell responses and so on (4). CrkL is a prominent substrate of the BCR/Abl oncoprotein in chronic myelogenous leukemia and binds to both BCR/Abl and c-Abl (5). CrkL is prominently and constitutively tyrosine phosphorylated in CML neutrophils and is not phosphorylated in normal neutrophils. Moreover, stimulation of normal neutrophils with cytokines and agonists does not induce tyrosine phosphorylation of this protein (6), indicating that it may be a useful target for therapeutic intervention or as a disease marker. Tyrosine 207 in CrkL is the BCR/Abl phosphorylation site (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hm Hu Rt

Synthetic peptide (KLH coupled) corresponding to N-terminal residues of human CrkL.

Detects endogenous levels of CrkL in various cell lines. It does not crossreact with any other related proteins. Species crossreactivity: Human, rat, hamster.

Purified by immunoaffinity chromatography

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol, less than 0.02% sodium azide.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 3i10(32H4) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol, less than 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide (KLH coupled) corresponding to N-terminal residues of human CrkL. Specificity: Detects endogenous levels of CrkL in various cell lines. It does not crossreact with any other related proteins. Species crossreactivity: Human, rat, hamster. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Satter, M., Salgia, R., Leukemia 12: 637-644 (1998). 2. Feller, S.M., et al., J. Cell. Physiol. 177: 535-552 (1998). 3. Kiyokawa, E., et al., Crit. Rev. Oncog. 8: 329-342 (1997). 4. Feller, S.M., et al., Oncogene 20: 6348-6371 (2001). 5. Grumbach, I.M., et al., Br. J. Haematol. 112: 327-336 (2001). 6. Nicholas, G.L., et al., Blood 84: 2912–291 (1994). 7. de Jong, R., et al., Oncogene 14: 507-513 (1997).

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