CREB (cAMP Response Element Binding Protein) | US Biological C7915-25 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CREB (cAMP Response Element Binding Protein)

catalog :

C7915-25

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

9B146(48H2)

reactivity :

human

product information

Catalog Number :

C7915-25

Product wo Prefix :

CREB (cAMP Response Element Binding Protein)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

9B146(48H2)

Isotype :

IgG

Desc1 :

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2. Applications: Suitable for use in Western Blot, Immunoprecipation, Chromatin Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 for 4ºC with gentle shaking overnight. Immunoprecipitation: 1:250 Chromatin Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:6400; Use citrate as an unmasking buffer. Immunohistochemistry (Frozen): 1:6400 Immunofluorescence (IC): 1:800 Immunofluorescence (Frozen): 1:800 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Dr Hu Mk Mo Rt

Immunogen :

Recombinant protein specific to the N-Terminal of human CREB protein

Specificity :

Recognizes endogenous levels of total human CREB protein at ~43kD. Species Crossreactivity: mouse, rat, monkey, D. melanogaster

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, <0.02% sodium azide

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 9B146(48H2) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, <0.02% sodium azide Purity: Purified Immunogen: Recombinant protein specific to the N-Terminla of human CREB protein Specificity: Recognizes endogenous levels of total human CREB protein at ~43kD. Species Crossreactivity: mouse, rat, monkey, D. melanogaster Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

ChIP FC IC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Lonze, B.E., et al., Neuron 34: 371-385 (2002). 2. Lee, M.M., et al., J. Neurosci. Res. 55: 702-712 (1999). 3. Redmond, L., et al., Neuron 34: 999-1010 (2002). 4. Dash, P.K., et al., Nature 345: 718-721 (1990). 5. Yin, J.C., et al., Cell 79: 49-58 (1994). 6. Guzowski, J.F. and McGaugh, J.L., Proc. Nat. Acad. Sci. USA 94: 2693-2698 (1997). 7. Xing, J., et al., Mol. Cell. Biol. 18: 1946-1955 (1998). 8. Ribar, T.J., et al., J. Neurosci. 20: RC107 (2000). 9. Tan, Y., et al., EMBO J. 15: 4629-4642 (1996).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/3/C7915-25_1.jpg

Picture4 Text :

Confocal immunofluorescent analysis of SK-N-MC cells showing nuclear stain with C7915-25 (A, red) compared to an isotype control (B). Blue pseudocolor = DRAQ5 (fluorescent DNA dye)

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/3/C7915-25_5.jpg

Picture5 Text :

Confocal immunofluorescent analysis of mouse cerebellum labeled with C7915-25 (red) and Neurofilament-L Mouse mAb (green). Blue pseudocolor = DRAQ5 (fluorescent DNA dye).

Picture 6 File Name :

https://usbio-images.r.worldssl.net/prodimages/3/C7915-25_6.jpg

Picture6 Text :

Flow Cytometric analysis of SK-N-MC cells using C7915-25 (blue) compared to a nonspecific negative control antibody (red).