antibody

CREB, phosphorylated (Ser133) (AlexaFluor®488)

C7915-04C

100 ul

monoclonal

domestic rabbit

AF488

phosphorylated

7H16(87G3)

CREB, phosphorylated (Ser133) (AlexaFluor®488)

rabbit

Mab

AlexaFluor®488

Phosphorylated

Antibodies

100 ul

7H16(87G3)

IgG

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations (1–3). Additionally, CREB signaling is involved in learning and memory in several organisms (4–6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (7–9). Applications: Suitable for use in Flow Cytometry and Immunofluorescence/Immunocytochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:50 Immunofluorescence (IC, Frozen): 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C after receipt. Freezing Alexa Fluor® conjugates will result in a substantial loss of activity. Alexa Fluor® conjugates are sensitive to light.

Hu Mo Rt

Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB. Species sequence homology: zebrafish

Recognize endogenous levels of human CREB only when phosphorylated at serine 133. Also detects the phosphorylated form of the CREB-related protein, ATF-1. Species Crossreactivity: mouse and rat

Purified by affinity chromatography.

Supplied as a liquid in PBS pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 488 (F/P ratio: 2-6).

Not determined

Product Type: Mab Isotype: IgG Clone No: 7H16(87G3) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in PBS pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with Alexa Fluor® 488 (F/P ratio: 2-6). Purity: Purified by affinity chromatography. Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB. Species sequence homology: zebrafish Specificity: Recognize endogenous levels of human CREB only when phosphorylated at serine 133. Also detects the phosphorylated form of the CREB-related protein, ATF-1. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC FL IC IF

4°C Do not freeze

1.Lonze, B.E. et al. (2002) Neuron 34: 371–385. 2. Lee, M.M. et al. (1999) J. Neurosci. Res. 55: 702–712. 3.Redmond, L. et al. (2002) Neuron 34: 999–1010. 4. Dash, P.K. et al. (1990) Nature 345: 718–721 5. Yin, J.C. et al. (1994) Cell 79: 49–58. 6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc. Nat. Acad. Sci. USA 94: 2693–2698. 7. Xing, J. et al. (1998) Mol. Cell. Biol. 18: 1946–1955. 8. Ribar, T.J. et al. (2000) J. Neurosci. 20: RC107. 9. Tan, Y. et al. (1996) EMBO J. 15: 4629–4642.

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