COX 1 (PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), Prostaglandin G/H synthase and cyclooxygenase) | US Biological C7904-73C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

COX 1 (PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), Prostaglandin G/H synthase and cyclooxygenase)

catalog :

C7904-73C

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C7904-73C

Product wo Prefix :

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000 Western Blot: 0.5-2ug/ml, ~60kD and 70kD bands observed in lysates of cell line HeLa (calculated MW of 64.5kD according to NP_542158.1 and 68.7kD according to NP_000953.2) Immunohistochemistry: 3-5ug/ml, in paraffin embedded Human Heart shows patchy staining of myocardial fibers in longitudinal section Optimal dilutions to be determined by the researcher. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See C7904-73C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to C- QDDGPAVERPSTEL from the C-terminus of human COX1. Species sequence homology: mouse.

Specificity :

Recognizes human COX1. This antibody is expected to recognize both human isoforms of this protein according to NP_000953.2 and NP_542158.1.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C- QDDGPAVERPSTEL from the C-terminus of human COX1. Species sequence homology: mouse. Specificity: Recognizes human COX1. This antibody is expected to recognize both human isoforms of this protein according to NP_000953.2 and NP_542158.1. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Yokoyama C, Tanabe T. , Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme., Biochem Biophys Res Commun. 1989 Dec 15;165(2):888-94.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/16/C7904-73C_1.jpg