Cortactin | US Biological C7901-83 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Cortactin

catalog :

C7901-83

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C7901-83

Product wo Prefix :

Cortactin

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Cortactin is a cortical actin binding protein. Its N-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acid that are important in F-actin binding and cross-linking. The C-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis. Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to the C-terminal residues of human cortactin (KLH).

Specificity :

Recognizes endogenous levels of human total cortactin protein. Species Crossreactivity: mouse, rat and monkey.

Purity :

Purified by protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the C-terminal residues of human cortactin (KLH). Specificity: Recognizes endogenous levels of human total cortactin protein. Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC WB

Storage Temperature :

-20°C

Reference :

1. Du, Y. et al. (1998) Mol. Cell. Biol. 18(10), 5838–5851. 2. Naisbitt, S. et al. (1999) Neuron 23, 569–582. 3. Rodrigo, J. P. et al. (2000) Clin. Cancer Res. 6, 3177–3182. 4. Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418–6434.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/5/C7901-83_1.jpg