Complement Factor B | US Biological C7850-60A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Complement Factor B

catalog :

C7850-60A

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H80

reactivity :

human

product information

Catalog Number :

C7850-60A

Product wo Prefix :

Complement Factor B

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

7H80

Isotype :

IgG2b

Desc1 :

Factor B is a serine protease of the alternative pathway of the complement system. The amino acid sequence of human factor B is 85% identical to that of rat and mouse. Applications: Suitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~5ng/lane under non-reducing conditions. Use of reducing conditions is not recommended. Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Factor B from conditioned cell culture medium. Direct ELISA: 0.5-1ug/ml; The detection limit is ~2ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant corresponding to aa26-764 of human Factor B expressed in NS0 cells (UniProt # P00751).

Specificity :

Recognizes recombinant human Complement Factor B. Does not crossreact with recombinant human Factor D or recombinant human Factor I. Species Sequence Homology: rat and mouse, 85%

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 7H80 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant corresponding to aa26-764 of human Factor B expressed in NS0 cells (UniProt # P00751). Specificity: Recognizes recombinant human Complement Factor B. Does not crossreact with recombinant human Factor D or recombinant human Factor I. Species Sequence Homology: rat and mouse, 85% Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C