Complement C3a, Human | US Biological C7850-13M product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Complement C3a, Human

catalog :

C7850-13M

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H121

reactivity :

human

product information

Catalog Number :

C7850-13M

Product wo Prefix :

Complement C3a, Human

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

7H121

Isotype :

IgG2b

Desc1 :

C3a is a 77 amino acid (aa) peptide comprising aa672-748 of the C3 precursor protein. It is generated as a proteolytic byproduct of complement activation. C3a is an anaphylatoxin that is vasoactive and mediates inflammatory responses. It binds G-protein coupled C3a receptors in airways during asthmatic allergen challenges, triggering robust mast cell degranulation. Human C3a shows 69% aa identity with mouse, rat and guinea pig C3a. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml. The detection limit is ~25ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Ser672-Arg748 from human C3a expressed in E. coli.

Specificity :

Recognizes human Complement C3a. Shows no crossreactivity with recombinant human C5a or recombinant human C3d.

Purity :

Purified by Protein G affinity chromatography from cell culture supernatant.

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 7H121 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein G affinity chromatography from cell culture supernatant. Immunogen: Recombinant protein corresponding to Ser672-Arg748 from human C3a expressed in E. coli. Specificity: Recognizes human Complement C3a. Shows no crossreactivity with recombinant human C5a or recombinant human C3d. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C