Cofilin, phosphorylated (Ser3) | US Biological C7506-20A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Cofilin, phosphorylated (Ser3)

catalog :

C7506-20A

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

7H15(77G2)

reactivity :

human

product information

Catalog Number :

C7506-20A

Product wo Prefix :

Cofilin, phosphorylated (Ser3)

Host :

rabbit

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H15(77G2)

Isotype :

IgG

Desc1 :

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration. In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments. The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin. Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm. Applications: Suitable for use in Western Blot and Immunofluorescence/ Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C after receipt. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Mk Mo Rt

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin.

Specificity :

Recognizes endogenous levels of human cofilin at ~19kD only when phosphorylated at serine 3. Species Crossreactivity: mouse, rat, monkey and bovine.

Purity :

Purified

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H15(77G2) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin. Specificity: Recognizes endogenous levels of human cofilin at ~19kD only when phosphorylated at serine 3. Species Crossreactivity: mouse, rat, monkey and bovine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF WB

Storage Temperature :

-20°C

Reference :

1. Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827–33830. 2. Condeelis, J. (2001) Trends Cell Biol. 11, 288–293. 3. Arber, S. et al. (1998) Nature 393, 805–809. 4. Yang, N. et al. (1998) Nature 393, 809–812. 5. Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449–31458. 6. Nebl, G. et al. (1996) J. Biol. Chem. 271, 26276–26280.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/37/C7506-20A_1.jpg