CLK3 (cdc2-like Kinase 3) | US Biological C5841-60 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CLK3 (cdc2-like Kinase 3)

catalog :

C5841-60

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C5841-60

Product wo Prefix :

CLK3 (cdc2-like Kinase 3)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The cdc2-like kinase (CLK) family contains at least four highly conserved isoforms CLK1, CLK2, CLK3 and CLK4 (1,2). CLKs are dual specific kinases that have been shown to autophosphorylate on serine, threonine and tyrosine residues and phosphorylate exogenous substrates on serine and threonine residues (2). CLK family members can co-exist as full-length catalytically active forms and an alternatively-spliced truncated inactive form (1). A family of highly phosphorylated proteins, so called serine- and arginine-rich (SR) proteins, have been shown to be phosphorylated by CLKs (3-5). SR proteins are splicing factors that regulate the assembly of the spliceosome, a macromolecular complex where RNA splicing occurs in the nucleus, and selection of splice sites. Thus, CLKs may play important roles in regulating RNA splicing. CLK3 is abundantly expressed in the testis and, similar to other family members, has been implicated in regulating RNA splicing (6-8). Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunofluorescence (IF-IC): 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding His51 of CLK3.

Specificity :

Detects endogenous levels of full-length and truncated forms of CLK3 protein. Species Crossreactivity: Human, monkey, mouse and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding His51 of CLK3. Specificity: Detects endogenous levels of full-length and truncated forms of CLK3 protein. Species Crossreactivity: Human, monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF WB

Storage Temperature :

-20°C

Reference :

1 Hanes, J. et al. (1994) J. Mol. Biol. 244, 665-672. 2 Nayler, O. et al. (1997) Biochem. J. 326, 693-700. 3 Colwill, K. et al. (1996) EMBO J. 15, 265-275. 4 Prasad, J. and Manley, J.L. (2003) Mol. Cell Biol. 23, 4139-4149. 5 Muraki, M. et al. (2004) J. Biol. Chem. 279, 24246-24254. 6 Menegay, H. et al. (1999) Exp. Cell Res. 253, 463-473. 7 Becker, W. et al. (1996) Biochim. Biophys. Acta 1312, 63-67. 8 Duncan, P.I. et al. (1998) Exp. Cell Res. 241, 300-308.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/26/C5841-60_1.jpg