Claudin 4 (CLDN4) | US Biological C5838-35C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Claudin 4 (CLDN4)

catalog :

C5838-35C

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7H177

reactivity :

human

product information

Catalog Number :

C5838-35C

Product wo Prefix :

Claudin 4 (CLDN4)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

7H177

Isotype :

IgG2a

Desc1 :

Claudin 4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin 4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also upregulated in several carcinomas. Phosphorylation of claudin 4 regulates paracellular epithelial permeability. Human claudin 4 shares 83% aa sequence identity with mouse and rat claudin 4. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 25ug/10e6 cells. Validated for use with PC-3 human prostate cancer cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Met1-Val209 from human Claudin 4 expressed in NS0 cells (NP_001296). Species Sequence Homology: mouse and rat, 83%

Specificity :

Recognizes human Claudin 4. Recognizes an epitope in the first extracellular loop (between aa 29-81). Stains human Claudin 4 transfectants but not irrelevant transfectants.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 7H177 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein A affinity chromatography. Immunogen: Recombinant protein corresponding to Met1-Val209 from human Claudin 4 expressed in NS0 cells (NP_001296). Species Sequence Homology: mouse and rat, 83% Specificity: Recognizes human Claudin 4. Recognizes an epitope in the first extracellular loop (between aa 29-81). Stains human Claudin 4 transfectants but not irrelevant transfectants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C