Chk2, phosphorylated (Ser33/35) (Checkpoint Kinase 2, Rad 53) | US Biological C4201-32 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Chk2, phosphorylated (Ser33/35) (Checkpoint Kinase 2, Rad 53)

catalog :

C4201-32

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

C4201-32

Product wo Prefix :

Chk2, phosphorylated (Ser33/35) (Checkpoint Kinase 2, Rad 53)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser33/35 of human Chk2.

Specificity :

Recognizes endogenous levels of human Chk2 only when phosphorylated at Ser33/35 at ~62kD. Does not crossreact with Chk2 phosphorylated at other sites. Species crossreactivity: monkey

Purity :

Purifie by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purifie by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser33/35 of human Chk2. Specificity: Recognizes endogenous levels of human Chk2 only when phosphorylated at Ser33/35 at ~62kD. Does not crossreact with Chk2 phosphorylated at other sites. Species crossreactivity: monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415. 2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665. 3 .Murakami, H. and Okayama, H. (1995) Nature 374, 817-819. 4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186. 5. Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394. 6. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765. 7. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936. 8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/C4201-32_1.jpg