Chk2, phosphorylated (Ser19) (Checkpoint Kinase 2, Rad 53) | US Biological C4201-30 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Chk2, phosphorylated (Ser19) (Checkpoint Kinase 2, Rad 53)

catalog :

C4201-30

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

C4201-30

Product wo Prefix :

Chk2, phosphorylated (Ser19) (Checkpoint Kinase 2, Rad 53)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Chk2 is the mammalian homologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4). Indeed, after DNA damage by ionizing radiation (IR), UV irradiation and DNA replication blocked by hydroxyurea, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser19 of human Chk2.

Specificity :

Detects endogenous levels of Chk2 only when phosphorylated at Ser19. The antibody does not crossreact with Chk2 phosphorylated at other sites.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser19 of human Chk2. Specificity: Detects endogenous levels of Chk2 only when phosphorylated at Ser19. The antibody does not crossreact with Chk2 phosphorylated at other sites. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC WB

Storage Temperature :

-20°C

Reference :

(1) Allen, J.B. et al. (1994) Genes Dev. 8, 2401–2415. (2) Weinert, T.A. et al. (1994) Genes Dev. 8, 652–665. (3) Murakami, H. and Okayama, H. (1995) Nature 374, 817–819. (4) Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179–186. (5) Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389–10394. (6) Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762–765. (7) Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934–5936. (8) Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537–30541.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/19/C4201-30_1.jpg