antibody

cdk9, p43 (PITALRE, cdc Kinase Family, Cyclin-dependent Kinase 9)

C2579-07

20 ul

monoclonal

domestic rabbit

nonconjugated

8A9(C12F7)

cdk9, p43 (PITALRE, cdc Kinase Family, Cyclin-dependent Kinase 9)

rabbit

Mab

Antibodies

20 ul

8A9(C12F7)

IgG

P-TEFb is a general transcription factor that regulates transcription elongation through phosphorylation of the C-terminal tail domain (CTD) of RNA polymerase II (RNAP II). The P-TEFb complex composed of a catalytic subunit, CDK9, and its regulatory cyclin partner, which can be cyclin T1, T2a, T2b or K (reviewed in 1,2). P-TEFb is recruited by the HIV Tat protein to allow transcriptional elongation, and subsequent replication of the viral genome. Inhibition of P-TEFb function therefore has potential for HIV therapy. CDK9 exists as two isoforms, an abundant 42kD isoform, and a less abundant 55kD isoform, which contains an amino-terminal extension (3). The two forms likely have distinct purposes based on differential expression during lymphocyte activation (4,5) and on their localization within the nucleus (5). Cyclin dependent kinases (CDKs) are activated in part by cyclin binding and by phosphorylation of a conserved threonine in the T-loop domain. Phosphorylation of CDK9 at the T-loop Thr186 by an unidentified nuclear kinase may be important in P-TEFb activation (6) and regulation of HIV transcription (7). Acetylation of CDK9 at Lys44 affects its ability to phosphorylate the RNAPII CTD (8). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Flow Cytometry Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (paraffin): 1:200 Immunohistochemistry (frozen): 1:200 Immunofluorescence (IF-IC): 1:100 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Ca Hm Hu Mk Mo Rt

Synthetic peptide corresponding to residues near the C-terminus of human CDK9.

Recognizes endogenous levels of total human CDK9 protein, both 42kD and 55kD isoforms. Species crossreactivity: canine, mouse, rat, monkey, hamster and bovine.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 8A9(C12F7) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the C-terminus of human CDK9. Specificity: Recognizes endogenous levels of total human CDK9 protein, both 42kD and 55kD isoforms. Species crossreactivity: canine, mouse, rat, monkey, hamster and bovine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IHC IP WB

-20°C

(1) Rice, A.P. and Herrmann, C.H. (2003) Curr HIV Res 1, 395–404. (2) De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342–7. (3) Shore, S.M. et al. (2003) Gene 307, 175–82. (4) Shore, S.M. et al. (2005) Gene 350, 51–8. (5) Liu, H. and Herrmann, C.H. (2005) J Cell Physiol 203, 251–60. (6) Chen, R. et al. (2004) J Biol Chem 279, 4153–60. (7) Ammosova, T. et al. (2005) Retrovirology 2, 47. (8) Fu, J. et al. (2007) Mol Cell Biol 27, 4641–51. Orders n 877-616-CELL (2355)

https://usbio-images.r.worldssl.net/prodimages/27/C2579-07_1.jpg

Flow cytometric analysis of Jurkat cells usin C2579-07g CDK9 (C12F7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

https://usbio-images.r.worldssl.net/prodimages/27/C2579-07_5.jpg

Confocal immunofluorescent analysis of HeLa cells using CDK9 (C12F7) Rabbit mAb (green). Actin filaments have been labeled with DY-555 phalloidin (red).