antibody

Cdc37 (S. cerevisiae hypothetical protein CDC37, CDC37 cell division cycle 37 homolog, Cdc37, Hsp90 chaperone protein kinase targeting subunit, Hsp90 p50Cdc37, co chaperone Cdc37, p50Cdc37)

C2562-04B

100 ul

monoclonal

domestic rabbit

nonconjugated

10E15(D28H7)

Cdc37 (S. cerevisiae hypothetical protein CDC37, CDC37 cell division cycle 37 homolog, Cdc37, Hsp90 chaperone protein kinase targeting subunit, Hsp90 p50Cdc37, co chaperone Cdc37, p50Cdc37)

rabbit

Mab

Antibodies

100 ul

10E15(D28H7)

IgG

CDC37 is an important component of the HSP90 chaperone complex (1,2). It was initially identified for its involvement in cell cycle progression and was later found to have a much broader role as a chaperone for a wide variety of kinases and other proteins (1-3). CDC37 protein has an amino-terminal kinase-binding domain followed by a central HSP90 binding domain. It recruits and stabilizes kinases in the HSP90 complex by protecting the newly synthesized kinase peptide chain from degradation and promoting the next step of protein maturation (4,5). CDC37 also suppresses the ATPase activity of HSP90, thereby leading to conformational changes in the complex that preclude target kinase loading (6). CDC37 has been proposed as a therapeutic target because of its important role in multiple kinase pathways involved in proliferation and cancer cell survival, including Raf, Akt, Src, ErbB2 pathways (7,8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to residues surrounding Val367 of human CDC37.

Recognizes endogenous levels of total human CDC37. Species crossreactivity: mouse, rat, monkey.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E15(D28H7) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Val367 of human CDC37. Specificity: Recognizes endogenous levels of total human CDC37. Species crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Karnitz, L.M. and Felts, S.J. (2007) Sci STKE 2007, pe22, 2. Caplan, A.J. et al. (2007) Trends Cell Biol 17, 87-92, 3. Caplan, A.J. et al. (2007) Cell Cycle 6, 3145-7, 4. Mandal, A.K. et al. (2007) J Cell Biol 176, 319-28, 5. Lee, P. et al. (2002) J Cell Biol 159, 1051-9, 6. Siligardi, G. et al. (2002) J Biol Chem 277, 20151-9, 7. Kimura, Y. et al. (1997) Genes Dev 11, 1775-85, 8. Gray, P.J. et al. (2008) Nat Rev Cancer 8, 491-5.

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