CDC25C, phosphorylated (Ser216) (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) | US Biological C2560-15 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

CDC25C, phosphorylated (Ser216) (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)

catalog :

C2560-15

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

C2560-15

Product wo Prefix :

CDC25C, phosphorylated (Ser216) (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). Cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

Cell Division Cycle 25C Protein, EC=3.1.3.48, Mitosis Inducer CDC25

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C.

Specificity :

Recognizes endogenous levels of human cdc25C at ~60kD only when phosphorylated at Ser216.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. Specificity: Recognizes endogenous levels of human cdc25C at ~60kD only when phosphorylated at Ser216. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

Ouyang, G. et al., (2009) Cell Biology International 33;1237-1244. 1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215–228. 2. Peng, C. Y. et al. (1997) Science 277, 1501–1505. 3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067–1072. 4. Blasina, A. et al. (1999) Curr. Biol. 9, 1–10. 5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833–845.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/19/C2560-15_1.jpg