antibody

CDC25C, phosphorylated (Thr48) (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)

C2560-05B

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

CDC25C, phosphorylated (Thr48) (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into the M-phase of the cell cycle (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). Ser216- phosphorylated cdc25C binds to members of the 14–3–3 family of proteins, sequestering cdc25C in the cytoplasm to prevent premature mitosis (3). The checkpoint kinases Chk1 and Chk2 have been shown to phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). Full activation of cdc25C involves phosphorylation at more than 12 different phosphorylation sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization on phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C have been shown to interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10). Recommended Dilution: Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Immunocytochemistry (for IF): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Cell Division Cycle 25C Protein, EC=3.1.3.48, Mitosis Inducer CDC25

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr48 of human cdc25C.

Phospho-cdc25C (Thr48) Antibody detects endogenous levels of cdc25C phosphorylated at threonine 48.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr48 of human cdc25C. Specificity: Phospho-cdc25C (Thr48) Antibody detects endogenous levels of cdc25C phosphorylated at threonine 48. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IC IF IHC WB

-20°C

(1) Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215–228. (2) Peng, C.Y. et al. (1997) Science 277, 1501–1505. (3) Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067–1072. (4) Blasina, A. et al. (1999) Curr. Biol. 9, 1–10. (5) Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833–845. (6) Izumi, T. and Maller, J.L. (1993) Mol. Biol. Cell 4, 1337–1350. (7) Stukenberg, P.T. et al. (2001) Mol. Cell 7, 1071–1083. (8) Yaffe, M.B. et al. (1997) Science 278, 1957–1960. (9) Lu, P.J. et al. (1999) Science 283, 1325–1328. (10) Landrieu, I. et al. (2001) J. Biol. Chem 276, 1434–1438.

https://usbio-images.r.worldssl.net/prodimages/6/C2560-05B_1.jpg