CD226 (CD226 Antigen, Adhesion Glycoprotein, DNAX Accessory Molecule 1, DNAM1, DNAM-1, Platelet and T cell Activation Antigen 1, PTA1, TLiSA1) | US Biological C2548-97G product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD226 (CD226 Antigen, Adhesion Glycoprotein, DNAX Accessory Molecule 1, DNAM1, DNAM-1, Platelet and T cell Activation Antigen 1, PTA1, TLiSA1)

catalog :

C2548-97G

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4j67 (DX11)

reactivity :

human

product information

Catalog Number :

C2548-97G

Product wo Prefix :

CD226 (CD226 Antigen, Adhesion Glycoprotein, DNAX Accessory Molecule 1, DNAM1, DNAM-1, Platelet and T cell Activation Antigen 1, PTA1, TLiSA1)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

4j67 (DX11)

Isotype :

IgG1

Desc1 :

CD226 is a 65kDa glycoprotein, also known as DNAM1 (DNAX accessory molecule-1). CD226 is broadly expressed on T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is also expressed by a subset of CD3 positive thymocytes. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Human cytotoxic T lymphocyte clone

Specificity :

Recognizes human CD226. Reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojima et al. 2003).

Purity :

Purified by Protein A affinity chromatography from tissue culture supernantant

Form :

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 4j67 (DX11) Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide Purity: Purified by Protein A affinity chromatography from tissue culture supernantant Immunogen: Human cytotoxic T lymphocyte clone Specificity: Recognizes human CD226. Reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojima et al. 2003). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IP

Storage Temperature :

-20°C

Reference :

1. Shibuya, A. et al. (1996) DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes. Immunity. 4: 573 - 581.2. Shibuya, A. et al. (1998) Protein kinase C is involved in the regulation of both signaling and adhesion mediated by DNAX Accessory molecule-1 receptor. J. Immunol. 161. 1671 - 1676. 3. Kojima, H. et al. (2003) CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells. J. Biol. Chem. 278 (38): 36748 - 36753.