antibody

CD122 (CD122 Antigen, High Affinity IL-2 Receptor beta Subunit, Interleukin 2 Receptor beta, Interleukin 2 Receptor Subunit beta, IL-2 Receptor, IL-2RB, IL2RB, P70-75, p75)

C2460-01M

100 ul

polyclonal

domestic rabbit

nonconjugated

CD122 (CD122 Antigen, High Affinity IL-2 Receptor beta Subunit, Interleukin 2 Receptor beta, Interleukin 2 Receptor Subunit beta, IL-2 Receptor, IL-2RB, IL2RB, P70-75, p75)

rabbit

Pab

Antibodies

100 ul

IgG

Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Ralpha, IL-2RBeta, and the common gamma chain, gammac (1,2). IL-2Ralpha binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2RB and gammac subunits. IL-15 also binds to IL-2Rbeta (1,2). Gammac is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to residues surrounding Thr420 of human IL-2RB.

Recognizes transfected levels of total human IL-2RB.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Thr420 of human IL-2RB. Specificity: Recognizes transfected levels of total human IL-2RB. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Ma, A. et al. (2006) Annu Rev Immunol 24, 657-79. 2. Gaffen, S.L. and Liu, K.D. (2004) Cytokine 28, 109-23. 3. Fehérvari, Z. et al. (2006) Trends Immunol 27, 109-11. 4. Antony, P.A. et al. (2006) J Immunol 176, 5255-66. 5. Jaleco, S. et al. (2003) J Immunol 171, 61-8.

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