antibody

CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) discontinued

C2448-20

100 ul

polyclonal

domestic rabbit

nonconjugated

CD117 (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) discontinued

rabbit

Pab

Antibodies

100 ul

IgG

c-Kit is the receptor tyrosine kinase for stem cell factor (SCF) and is a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors (1,2). It plays critical controlling roles in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells (3). Upon binding with its ligand, c-Kit receptors are induced undergoing dimerization/ oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCgamma, p85 subunit of PI3 kinase, SHP2 and CrkL (4), which links c-Kit directly to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyrosine 719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to the residues surrounding Met720 of human c-Kit (KLH).

Recognizes endogenous levels of human c-Kit at 120 and 145kD. Does not crossreact with any other receptor tyrosine kinase family members.

Purified by Protein A and Peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and Peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to the residues surrounding Met720 of human c-Kit (KLH). Specificity: Recognizes endogenous levels of human c-Kit at 120 and 145kD. Does not crossreact with any other receptor tyrosine kinase family members. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Martin, F.H., et al., Cell 63: 203–211 (1990). 2. Yarden, Y., et al., EMBO J. 6: 3341–3351 (1987). 3. Gommerman, J.L., et al., J. Biol. Chem. 272: 30,519–30,525 (1997). 4. Sattler, M., et al., J. Biol. Chem. 272: 10,248–10,253 (1997). 5. Nocka, K., et al., EMBO J. 9: 1805–1813 (1990). 6. Hirota, S., et al., Science 279: 577–580 (1998). 7. Blume-Jensen, P., et al., Nat. Genet. 24: 157–162 (2000).

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