antibody

CD117, CT (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)

C2448-04N

100 ul

monoclonal

mouse

nonconjugated

Ab81

The same clone is also sold as:

• Cell Signaling Technology: 3308, 3310

CD117, CT (c-kit, Stem Cell Factor, SCF Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF)

mouse

Mab

Antibodies

100 ul

Ab81

IgG1,k

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1 and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells (3). Upon binding with its ligand, stem cell factor (SCF), c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLC gamma, the p85 subunit of PI3 kinase, SHP2 and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot 1:1000 Immunoprecipitation 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Recombinant proteins containing the cytoplasmic domain of human c-Kit.

Detects endogenous levels of total c-Kit protein. It does not crossreact with other receptor tyrosine kinase family members. Species Crossreactivity: Human.

Ascites

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

Not determined

Product Type: Mab Isotype: IgG1,k Clone No: Ab81 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide. Purity: Ascites. Immunogen: Recombinant proteins containing the cytoplasmic domain of human c-Kit. Specificity: Detects endogenous levels of total c-Kit protein. It does not crossreact with other receptor tyrosine kinase family members. Species Crossreactivity: Human. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IP WB

-20°C

(1) Martin, F.H. et al. (1990) Cell 63, 203–211. (2) Yarden, Y. et al. (1987) EMBO J. 6, 3341–3351. (3) Gommerman, J.L. et al. (1997) J. Biol. Chem. 272, 30519–30525. (4) Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248–10253. (5) Nocka, K. et al. (1990) EMBO J. 9, 1805–1813. (6) Hirota, S. et al. (1998) Science 279, 577–580. (7) Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157–162.

https://usbio-images.r.worldssl.net/prodimages/35/C2448-04N_1.jpg