antibody

CD115, phosphorylated (Tyr809) (c-fms, Fms, CSF-1R, M-CSFR)

C2447-49

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

CD115, phosphorylated (Tyr809) (c-fms, Fms, CSF-1R, M-CSFR)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the c-fms proto-oncogene. MCSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyrosine 723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated tyrosine 723 binds the p85 subunit of PI3 kinase as well as PLC-gamma2 (5). Phosphorylation of Tyr809 in the receptor provides a docking site for Shc adaptor protein (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic peptide corresponding to residues around Tyr809 of human M-CSF receptor.

Recognizes endogenous levels of human M-CSF receptors at ~175kD only when phosphorylated at Tyr809. Slight crossreactivity with activated KDR and PDGF receptor. Does not crossreact with other unrelated tyrosine-phosphorylated proteins. Species Crossreactivity: mouse.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues around Tyr809 of human M-CSF receptor. Specificity: Recognizes endogenous levels of human M-CSF receptors at ~175kD only when phosphorylated at Tyr809. Slight crossreactivity with activated KDR and PDGF receptor. Does not crossreact with other unrelated tyrosine-phosphorylated proteins. Species Crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Stanley, E.R., et al., Nature 274: 168-170 (1978). 2. Byrne, P.V., et al., J. Cell. Biol. 91: 848-853 (1981). 3. Bourette, R.P., et al., Growth Factors 17: 155-166 (2000). 4. Novak, U., et al., Oncogene 13: 2607-2613 (1996). 5. Bourette, R.P., et al., EMBO J. 16: 5880-5893 (1997). 6. Morley, G.M., et al., Oncogene 18: 3076-3084 (1999). 7. Toy, E.P., et al., Gynecol. Oncol. 80: 194-200 (2001). 8. Maher, M.G., et al., Clin. Cancer Res. 4: 1851-1856 (1998).

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