antibody

CD105 (Endoglin, gp160, TGF beta1,3 Receptor, HHT1, ORW1, Osler Rendu Weber Syndrome 1)

C2446-50E5

100 ul

monoclonal

domestic rabbit

nonconjugated

10E14(D50G1)

CD105 (Endoglin, gp160, TGF beta1,3 Receptor, HHT1, ORW1, Osler Rendu Weber Syndrome 1)

rabbit

Mab

Antibodies

100 ul

10E14(D50G1)

IgG

Endoglin (ENG, CD105) is an auxiliary receptor for the TGF-Beta receptor complex, functioning in related signaling pathways (1,2). Endoglin is a transmem-brane protein that exists as a disulfide-linked homodimer. It is mainly expressed in vascular and connective tissues and in endothelial and stromal cells. Upregulated endoglin expression has been reported during wound healing and tumor vascularization, and in inflammatory tissues and developing embryos (1-4). Mutations in endoglin have been found to be a causal factor of hereditary hemorrhagic telangiectasia (HHT), a disease characterized by malformation of vascular structure (5,6). The importance of this protein for normal and tumor vascular function makes it a good marker for endothelial cell proliferation as well as a potential therapeutic target in cancer (4-6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to residues surrounding Ala463 of human endoglin protein.

Recongnizes endogenous levels of human total endoglin protein.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E14(D50G1) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Ala463 of human endoglin protein. Specificity: Recongnizes endogenous levels of human total endoglin protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Bernabeu, C. et al. (2007) J Cell Biochem 102, 1375-88, 2. Zhu, Y. et al. (2003) Stroke 34, 2483-8, 3. Quintanilla, M. et al. (2003) Oncogene 22, 5976-85, 4. Dallas, N.A. et al. (2008) Clin Cancer Res 14, 1931-7, 5. ten Dijke, P. et al. (2008) Angiogenesis 11, 79-89, 6. Fernández-L, A. et al. (2006) Clin Med Res 4, 66-78

https://usbio-images.r.worldssl.net/prodimages/27/C2446-50E5_1.jpg