antibody

CD95 (Fas)

C2442-21E

20 ul

polyclonal

domestic rabbit

nonconjugated

CD95 (Fas)

rabbit

Pab

Antibodies

20 ul

IgG

Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenophathy and splenomegaly as a result of decreased apoptosis in CD4-CD8-T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type 2 membrane protein of 280aa (runs at ~40kD upon glycosylation) that belongs to the TNF family which also includes TNF-alpha, TRAIL and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to the execution of apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells and at immunologically privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26kD trimeric soluble form (8,9). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:480 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to residues surrounding Lys259 of human Fas, within the intracellular region.

Recognizes endogenous levels of total human Fas protein.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Lys259 of human Fas, within the intracellular region. Specificity: Recognizes endogenous levels of total human Fas protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IHC WB

-20°C

1. Suda, T. et al. (1993) Cell 75:1169–78. 2. Lee, H.O. and Ferguson, T.A. (2003) Cytokine Growth Factor Rev 14:325–35. 3. Watanabe-Fukunaga, R. et al. (1992) Nature 356:314–7. 4. Hahne, M. et al. (1995) Int Immunol 7:1381–6. 5. Nagata, S. (1997) Cell 88:355–65. 6. Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2:917–24. 7. Walker, P.R. et al. (1997) J Immunol 158:4521–4. 8. Kayagaki, N. et al. (1995) J Exp Med 182:1777–83. 9. Tanaka, M. et al. (1995) EMBO J 14:1129–35.

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