antibody

CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7)

C2432-03E

100 ug

polyclonal

domestic goat

nonconjugated

CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7)

goat

Pab

Antibodies

100 ug

IgG

CD80 (B7-1) is a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:4000 Western Blot: 0.3-1ug/ml, observed in human lymph node and tonsil lysates on ~60kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic peptide corresponding to C-HLRVNQTFNWNTTK, from human CD80, at the internal region of the protein (NP_005182.1).

Recognizes human CD80.

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-HLRVNQTFNWNTTK, from human CD80, at the internal region of the protein (NP_005182.1). Specificity: Recognizes human CD80. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Stephan MT, Ponomarev V, Brentjens RJ, Chang AH, Dobrenkov KV, Heller G, Sadelain M, T cell-encoded CD80 and 4-1BBL induce auto- and transcostimulation, resulting in potent tumor rejection. Nature medicine 2007 Dec 13(12):1440-9.

https://usbio-images.r.worldssl.net/prodimages/12/C2432-03E_1.jpg