antibody

CD70 (CD27L, CD27-L, CD27LG, CD27 ligand, CD70 antigen, TNFSF7, Tumor necrosis factor ligand superfamily member 7

C2425-01B

100 ug

polyclonal

domestic goat

nonconjugated

CD70 (CD27L, CD27-L, CD27LG, CD27 ligand, CD70 antigen, TNFSF7, Tumor necrosis factor ligand superfamily member 7

goat

Pab

Antibodies

100 ug

IgG

Human CD27 Ligand (also CD70) is a 30kD type 2 transmembrane glycoprotein that is a member of the TNF superfamily. It contains a 20aa cytoplasmic region and a 155aa extracellular domain that shows multiple b-strands. It is an inducible trimer that occurs on B cells, T cells and NK cells. It regulates T cell dependant B cell differentiation into plasma cells and likely to promotes clonal expansion of T cells. Human CD27 Ligand extracellular region is 64% aa identical to mouse and rat CD27 Ligand extracellular regions. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using THP-1 human acute monocytic leukemia cell line Flow Cytometry: 2.5ug labels 10e6 Raji cells from human Burkitt’s lymphoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Recombinant protein corresponding to Gln45-Pro193 from human CD70 Ligand expressed in NS0 cells.

Recognizes human CD70 Ligand. Crossreactivity: ~10% with recombinant mouse CD70 Ligand and recombinant human CD30 Ligand in ELISA. Shows <1% crossreactivity with recombiniant human CD40 Ligand.

Purified by immunoaffinity chromatography

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

~0.2mg/ml (after reconstitution)

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography Immunogen: Recombinant protein corresponding to Gln45-Pro193 from human CD70 Ligand expressed in NS0 cells. Specificity: Recognizes human CD70 Ligand. Crossreactivity: ~10% with recombinant mouse CD70 Ligand and recombinant human CD30 Ligand in ELISA. Shows <1% crossreactivity with recombiniant human CD40 Ligand. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/30/C2425-01B_1.jpg