CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (FITC) | US Biological C2409-48H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (FITC)

catalog :

C2409-48H

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

1A29

The same clone is also sold as:

Bio-Rad: MCA773F, MCA773, MCA773GA
Novus Biologicals: NBP2-22541-0.1mg
BD Biosciences: 554967, 554970, 554966
BioLegend: 202403, 202405
OriGene: SM286FX, SM286PS, SM286F, SM286LE, SM286P
Invitrogen: MA5407, 46-0540-80
Abcam: ab171123
Santa Cruz Biotechnology: sc-19584, sc-19584 L

reactivity :

rat

product information

Catalog Number :

C2409-48H

Product wo Prefix :

CD54 (BB2, Cell Surface Glycoprotein P3.58, Human Rhinovirus Receptor, Intercellular Adhesion Molecule 1, ICAM 1, ICAM-1, Major Group Rhinovirus Receptor, MALA-2, MyD10, P3.58) (FITC)

Host :

mouse

Product Type :

Mab

Conjugate :

FITC

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

1A29

Isotype :

IgG1

Desc1 :

CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation. CD29 is reported to inhibit beta 1 integrin mediated adhesion (1). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:100, Use 10ul to label 10e6 cells in 100ul. Optimal dilution determined by the researcher Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells from the PAI mouse myeloma cell line. Recommended Negative Controls: IgG1 Negative Control: FITC Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Ax cells (a rat HEV derived cell line).

Specificity :

Recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), which is a 90kD adhesion molecule belonging to the immunoglobulin superfamily. This is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system.

Purity :

Purified by Protein G affinity chromatography from tissue culture supernatant.

Form :

Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 1A29 Host: mouse Source: rat Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC). Purity: Purified by Protein G affinity chromatography from tissue culture supernatant. Immunogen: Ax cells (a rat HEV derived cell line). Specificity: Recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), which is a 90kD adhesion molecule belonging to the immunoglobulin superfamily. This is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Tamatani, T. and Miyasaka, M. (1990). Identification of monoclonal antibodies reactive with the rat homologue of ICAM-1, and evidence for a differential involvement of ICAM-1 in the adherence of resting versus activated lymphocytes to high endothelial cells. Int. Immunol. 2: 165. 2. Tamatani, T. et al. (1991). Molecular mechanisms underlying lymphocyte recirculation. II. Differential regulation of LFA-1 in interaction between lymphocytes and high endothelial cells. Eur. J. Immunol. 21: 855-858.