antibody

CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)

C2398-05B

100 ul

monoclonal

mouse

nonconjugated

10E13(8E2)

CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1)

mouse

Mab

Antibodies

100 ul

10E13(8E2)

IgG1

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:1600 Flow Cytometry: 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Rt

Recombinant protein corresponding to CD44.

Recognizes endogenous levels of total human CD44. Species crossreactivity: rat.

Purified

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 8E2 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Recombinant protein corresponding to CD44. Specificity: Recognizes endogenous levels of total human CD44. Species crossreactivity: rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IP WB

-20°C

1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196, 2. Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843, 3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913- 27918, 4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407, 5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895, 6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.

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