CD42b alpha (Glycoprotein Ib alpha, GPIba) | US Biological C2395-20B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD42b alpha (Glycoprotein Ib alpha, GPIba)

catalog :

C2395-20B

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8H211

reactivity :

human

product information

Catalog Number :

C2395-20B

Product wo Prefix :

CD42b alpha (Glycoprotein Ib alpha, GPIba)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

8H211

Isotype :

IgG1

Desc1 :

GPIb-alpha (platelet glycoprotein Ib-alpha; also CD42b and glycocalicin) is a platelet-derived 145kD member of the leucine-rich glycoprotein family of molecules. Mature human GPIb-alpha is a type I transmembrane protein 610 amino acids (aa) in length. It contains a 489 aa extracellular domain (ECD) (aa 17-505) and a 100 aa cytoplasmic region (aa 527-626). As part of a membrane complex, the GPIb-alpha ECD binds to von Willebrand Factor to impede platelet flow and promote clotting. The human GPIb-alpha ECD is approximately 50% aa identical to dog and mouse GPIb-alpha ECD. Applications: Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells using human CD41+ platelets Western Blot: 2ug/ml using human platelets Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Alternate Name :

Glycocalicin

Immunogen :

Recombinant protein corresponding to His17-Leu505 from human CD42b alpha, expressed in NS0 cells.

Specificity :

Recognizes human CD42b alpha.

Purity :

Purified by Protein G affinity chromatography from cell culture supernatant.

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 8H211 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein G affinity chromatography from cell culture supernatant. Immunogen: Recombinant protein corresponding to His17-Leu505 from human CD42b alpha, expressed in NS0 cells. Specificity: Recognizes human CD42b alpha. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC WB

Storage Temperature :

-20°C

Reference :

1. Andrews, R.K. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1511. 2. Lopez, J.A. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5615. 3. Wenger, R.H. et al. (1988) Biochem. Biophys. Res. Commun. 156:389. 4. Luo, S-Z. et al. (2007) Blood 109:603. 5. Uff, S. et al. (2002) J. Biol. Chem. 277:35657. 6. Shen, Y. et al. (2006) J. Biol. Chem. 281:26419. 7. Ishida, F. et al. (1995) Blood 86:1356. 8. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530. 9. Beer, J.H. et al. (1994) Blood 83:691. 10. Adam, F. et al. (2003) Eur. J. Biochem. 270:2959. 11. Baglia, F.A. et al. (2004) J. Biol. Chem. 279:49323. 12. Bradford, H.N. et al. (2000) J. Biol. Chem. 275:22756. 13. Wang, Y. et al. (2005) Circulation 112:2993. 14. Othman, M. et al. (2005) Blood 105:4330.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/11/C2395-20B_1.png