CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1) | US Biological C2386-12 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)

catalog :

C2386-12

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

pigs

product information

Catalog Number :

C2386-12

Product wo Prefix :

CD34 (Endothelial Cell Marker, Cluster Designation 34, Hematopoietic Progenitor Cell Antigen CD34, HPCA1)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

CD34 is a 110kD type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Porcine CD34 is 389aa in length and is predicted to have a 272aa extracellular domain (ECD) and a 73aa cytoplasmic tail. An alternate 307aa splice form with a 4aa substitution for the C-terminal 61aa also exists. Over aa20-294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively. Applications: Suitable for use in Western Blot and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Ala20-Thr294 from the extracellular domain of porcine CD34 expressed in NSO cells with Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala and His278Asn substitutions

Specificity :

Recognizes the extracellular domain of porcine CD34. Shows ~20% crossreactivity with rrCD34 and <5% with rmCD34 and rhCD34.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Concentration :

~0.2mg/ml (after reconstitution)

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: porcine Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography Immunogen: Recombinant protein corresponding to Ala20-Thr294 from the extracellular domain of porcine CD34 expressed in NSO cells with Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala and His278Asn substitutions Specificity: Recognizes the extracellular domain of porcine CD34. Shows ~20% crossreactivity with rrCD34 and <5% with rmCD34 and rhCD34. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Kitaura-Inenaga K, Hara M, Higuchi K, Yamamoto K, Yamaki A, Ono K, Nakano A, Kinoshita M, Sasayama S, Matsumori A. Gene expression of cardiac mast cell chymase and tryptase in a murine model of heart failure caused by viral myocarditis. Circ. J. 2003 Oct 67 (10): 881-4. PMID: 14578624.