CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein) | US Biological C2265-32P1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein)

catalog :

C2265-32P1

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3H501 (TÜK4)

reactivity :

human

product information

Catalog Number :

C2265-32P1

Product wo Prefix :

CD14 (CD14 Antigen, Lipopolysaccharide Receptor, LPSR, Monocyte Differentiation Antigen 14, Myeloid Cell Specific Leucine Rich Glycoprotein)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

3H501 (TÜK4)

Isotype :

IgG2a

Desc1 :

CD14 is a ~55 kD glycoprotein that contains multiple leucine-rich repeats. It is anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) linkage (Simmons et al. 1989), a soluble form of CD14 also exists (Bazil et al. 1986). CD14 is strongly expressed on the surface of monocytes and macrophages but has also been shown to be expressed on the surface of non-myeloid cells (Jersmann 2005). CD14 functions as a pattern recognition receptor (Pugin et al. 1994, Dziarski et al. 1998) in innate immunity for a variety of ligands, in particular for the LPS (endotoxin) of Gram-negative bacteria. C2265-32P1 has been shown to block SDF-induced chemotaxis of U937 cells in a dose –dependent manner (Yang et al. 2003). Applications: Suitable for use in ELISA, Flow Cytometry, Functional Assays, and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Ca Fe Gt Hu Mk Po Rb Sh

Specificity :

Recognizes human CD14. Species Crossreactivity: canine, goat, feline, rabbit, mink, bovine, porcine, sheep, Cynomolgus monkey and llama.

Purity :

Purified by Protein A affinity chromatography from tissue culture supernatant. Endotoxin: <0.01EU/ug

Form :

Supplied as a liquid in PBS. No preservative added.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 3H501 (TÜK4) Host: mouse Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS. No preservative added. Purity: Purified by Protein A affinity chromatography from tissue culture supernatant. Endotoxin: <0.01EU/ug Specificity: Recognizes human CD14. Species Crossreactivity: canine, goat, feline, rabbit, mink, bovine, porcine, sheep, Cynomolgus monkey and llama. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC WB

Storage Temperature :

-20°C

Reference :

1. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49 (2): 97-110, 2. Soell, M et al. (1995) Activation of human monocytes by streptococcal rhamnose glucose polymers is mediated by CD14 antigen, and mannan binding protein inhibits TNF-alpha release. J. Immunol. 154: 851-860, 3. Gupta, V.K. et al. (1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14. Vet Immunol. Immunopathol. 51: 89-99, 4. Jacobsen, C.N. et al. (1993) Reactivities of 20 anti-human monoclonal antibodies with leucocytes from ten different animal species. Vet. Immunol. Immunopathol. 39: 461-466, 5. Sopp, P. et al. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells. Vet. Immunol. Immunopathol. 56: 11-25, 6. Arriens, M.A. et al. (1996) Cross reactivity of anti-human CD monoclonal antibodies with porcine leukocytes. Vet. Immunol. Immunopathol. 54: 113, 7. Werling, D et al. (1998) Analysis of the phenotype and phagocytic activity of monocytes/macrophages from cattle infected with the bovine leukaemia virus. Vet. Immunol. Immunopathol. 62:185-195, 8. Willett, B. et al. (2003) Expression of CXCR4 on feline peripheral blood mononuclear cells: effect of feline immunodeficiency virus infection. J. Virol. 77: 709 - 712, 9. Yang, H. et al. (2003) Antibody to CD14 like CXCR4-specific antibody 12G5 could inhibit CXCR4-dependent chemotaxis and HIV Env-mediated cell fusion. Immunol. Letters 88: 27-30.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/35/C2265-32P1_1.jpg