CD14 (CD14 antigen, CD14 molecule, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein) | US Biological C2265-31H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD14 (CD14 antigen, CD14 molecule, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein)

catalog :

C2265-31H

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8B7

reactivity :

pigs

product information

Catalog Number :

C2265-31H

Product wo Prefix :

CD14 (CD14 antigen, CD14 molecule, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

8B7

Isotype :

IgG2b

Desc1 :

CD14 is a 55kD GPI-linked cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. It is a pattern recognition protein that acts as a receptor for lipopolysaccharide (LPS) and other microbial cell wall components. CD14 also occurs as a secreted protein. Applications: Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications have not been tested Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit is ~25ng/lane under non-reducing and reducing conditions. Flow Cytometry: 25ug/ml. Add 10ul to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. Direct ELISA: 0.5-1ug/ml. The detection limit is~1ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to aa44-175 from porcine CD14 expressed in E. coli. UniProt Accession: Q95122. Species Sequece Homology: human, bovine, sheep, water buffalo and equine: 70-75%, mouse and rat: 61-62%.

Specificity :

Recognizes porcine CD14. Does not crossreact with recombinant human CD14 or recombinant mouse CD14 in Western Blot and DIrect ELISA.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

Concentration :

~0.5mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2b Clone No: 8B7 Host: mouse Source: porcine Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity: Purified by Protein A affinity chromatography. Immunogen: Recombinant protein corresponding to aa44-175 from porcine CD14 expressed in E. coli. UniProt Accession: Q95122. Species Sequece Homology: human, bovine, sheep, water buffalo and equine: 70-75%, mouse and rat: 61-62%. Specificity: Recognizes porcine CD14. Does not crossreact with recombinant human CD14 or recombinant mouse CD14 in Western Blot and DIrect ELISA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC WB

Storage Temperature :

-20°C