antibody

Caveolin 1, phosphorylated (Tyr14) (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)

C2097-93A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Caveolin 1, phosphorylated (Tyr14) (CAV1, caveolae protein, 22kD , HGNC:1527, CAV, MSTP085, VIP21, caveolin 1, alpha isoform, caveolin 1, beta isoform, cell growth-inhibiting protein 32)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The 21-24kD integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of caveolins (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero-and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis. It is also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as G-alpha subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Tyrosine 14 has been identified as one of the tyrosine phosphorylation sites on caveolin-1 (3,4). Phosphorylation at Tyr14 is essential for its association with SH2 or PTB domaincontaining adaptor proteins, such as GRB7 (5). Phosphorylation at serine 80 is a key regulatory event for its binding to the ER membrane and entry into the secretory pathway (6). Applications: Suitable for use in Western Blot Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr14 of human caveolin-1.

Detects endogenous levels of caveolin-1 phosphorylated at tyrosine 14. Does not crossreact with caveolin-2, -3 or caveolin-1beta, the short isoform of caveolin-1. Species Crossreactivity: human, mouse, rat and monkey

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr14 of human caveolin-1. Specificity: Detects endogenous levels of caveolin-1 phosphorylated at tyrosine 14. Does not crossreact with caveolin-2, -3 or caveolin-1beta, the short isoform of caveolin-1. Species Crossreactivity: human, mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

(1) Okamoto, T. et al. (1998) J. Biol. Chem. 273, 5419–5422. (2) Smart, E.J. et al. (1999) Mol. Cell. Biol. 19, 7289–7304. (3) Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975–986. (4) Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094–8103. (5) Lee, H. et al. (2000) Mol. Endocrinol. 14, 1750–1775. (6) Schlegel, A. et al. (2001) J. Biol. Chem. 276, 4398–4408.

https://usbio-images.r.worldssl.net/prodimages/26/C2097-93A_1.jpg