Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal Aspartyl Peptidase) | US Biological C2097-24C product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal Aspartyl Peptidase)

catalog :

C2097-24C

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C2097-24C

Product wo Prefix :

Cathepsin D (CatD, CLN10, CPSD, CTSD, Lysosomal Aspartyl Peptidase)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Cathepsin D is a ubituitiously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43kD preprocathepsin D that is cleaved and glycosylated to form a 46kD procathepsin D and then further cleaved to produce 28kD and 15kD subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularily in breast cancer (4-9). Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:200 Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk

Immunogen :

Synthetic peptide corresponding to residues with the heavy chain subunit of human Cathepsin D.

Specificity :

Recognizes endogenous levels of human preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D. Species Crossreactivity: monkey

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues with the heavy chain subunit of human Cathepsin D. Specificity: Recognizes endogenous levels of human preprocathepsin D, procathepsin D and the heavy chain subunit of mature cathepsin D. Species Crossreactivity: monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IHC WB

Storage Temperature :

-20°C

Reference :

1) Faust, P.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 4910–4914. (2) Erickson, A.H. et al. (1981) J. Biol .Chem. 256, 11224–11231. (3) Liaudet-Coopman, E. et al. (2006) Cancer Lett. 237, 167–179. (4) Berchem, G. et al. (2002) Oncogene 21, 5951–5955. (5) Nomura, T. and Katunuma, N. (2005) J. Med. Invest. 52, 1–9. (6) Garcia, M. et al. (1996) Stem Cells 14, 642–560. (7) Nogami, M. et al. (2000) Histochem. J. 32, 505–508. (8) Nakanishi, H. (2003) Ageing Res. Rev. 2, 367–381. (9) Callahan, L.M. et al. Neurobiol. Aging 19, S99–105.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/3/C2097-24C_1.jpg