Catenin, beta | US Biological C2096-60 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Catenin, beta

catalog :

C2096-60

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C2096-60

Product wo Prefix :

Catenin, beta

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3 beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues around Ser37 of human beta-catenin.

Specificity :

Recognize endogenous levels total of human beta-catenin. Does cross-react with endogenous levels of γ-catenin. Species Crossreactivity: mouse, monkey and rat.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues around Ser37 of human beta-catenin. Specificity: Recognize endogenous levels total of human beta-catenin. Does cross-react with endogenous levels of γ-catenin. Species Crossreactivity: mouse, monkey and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Cardigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286–3305. 2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell Dev. Biol. 14, 59–88. 3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15–21. 4. Yost, C. et al. (1996) Genes Dev. 10, 1443–1454. 5. Morin, P.J. (1997) Science 275, 1787–1790.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/41/C2096-60_1.jpg